Plant Cell Wall Imaging by Metabolic Click-Mediated Labelling of Rhamnogalacturonan II Using Azido 3-deoxy-D-manno-oct-2-ulosonic acid.
In plants, 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) is a monosaccharide that is only found in the cell wall pectin, rhamnogalacturonan-II (RG-II). Incubation of 4-day-old light-grown Arabidopsis seedlings or tobacco BY-2 cells with 8-azido 8-deoxy Kdo (Kdo-N3) followed by coupling to an alkyne containing fluorescent probe resulted in the specific in muro labelling of RG-II through a copper-catalyzed azide–alkyne cycloaddition reaction. CMP-Kdo synthetase inhibition and competition assays showing that Kdo and
Additionally, after incubating plants with Kdo-N3 and an alkynated derivative of