An Atlas of Human Glycosylation Pathways Enables Display of the Human Glycome by Gene Engineered Cells

By genetically engineering the cellular glycosylation machinery, a cell-based glycan array has been constructed. Maps of the glycosylation pathway were used to generate a library of isogenic HEK293 cells that display combinatorially engineered glycosylation capacities. This array, which is self-renewable, presents the human glycome in a natural glycoconjugate and cell-surface context. Several examples demonstrate the broad utility of the cell-based glycan array. The presentation of a substantial part of the human glycome opens the road to a new a deeper understanding of the informational content and biological roles of the human glycome.