High-Throughput Glycomics and Glycoproteomics Methods and Protocols

The analysis of glycans is an often challenging endeavor, due to their heterogeneity, their non-template-based synthesis, and the abundance of functionally distinct isomers which are challenging for separation and characterisation using current analytical techniques.

Technological challenges in glycan analysis are generally seen as the major obstacle for revealing insights into functional relevance of glycans. Several robust methods that enable high-throughput analysis of glycans have been developed. Glycomics is now ready to join genomics and other omics in genetic, epidemiological, and clinical studies. This volume of Methods in Molecular Biology entitled High-Throughput Glycomics and Glycoproteomics brings detailed protocols for the analysis of glycosylation at the level of free glycans and glycopeptides, an essential reference for researchers planning to enter this rapidly evolving field.

• Ubiquitous Importance of Protein Glycosylation, J. Krištić & G. Lauc
• Laboratory Experimental Design for a Glycomic Study, I. Ugrina, H. Campbell & F. Vučković
• High-Throughput Analysis of the IgG N-Glycome by UPLC-FLR, M. Pučić-Baković
• High-Throughput Analysis of IgG Fc Glycopeptides by LC-MS, D. Falck, B.C. Jansen, N. de Haan, M. Wuhrer
• Sialic Acid Derivatization for the Rapid Subclass- and Sialic Acid Linkage-Specific MALDI-TOF-MS Analysis of IgG Fc-Glycopeptides, N. de Haan, K.R. Reiding & M. Wuhrer
• The Use of Multiple Reaction Monitoring on QQQ-MS for the Analysis of Protein- and Site-Specific Glycosylation Patterns in Serum, L. R. Ruhaak
• Analysis of Permethylated Glycan by Liquid Chromatography (LC) and Mass Spectrometry (MS), S. Zhou, K.M. Wooding & Y. Mechref
• High-Throughput Analysis of the Plasma N-Glycome by UHPLC, B. Adamczyk, H. Stöckmann, R. O’Flaherty & N.G. Karlsson…
• Site-Specific N- and O-Glycopeptide Analysis Using an Integrated C18-PGC-LC-ESI-QTOF-MS/MS Approach, K. Stavenhagen, H. Hinneburg, D. Kolarich, M. Wuhrer
• Analysis of Milk Oligosaccharides by Mass Spectrometry, L.D. Wu, L.R. Ruhaak, & B. Lebrilla
• N- and O-Glycomics from Minor Amounts of Formalin-Fixed, Paraffin-Embedded Tissue Samples, H. Hinneburg, F. Schirmeister, P. Korać & D. Kolarich
• Carbohydrate Microarray Technology Applied to High-Throughput Mapping of Plant Cell Wall Glycans Using Comprehensive Microarray Polymer Profiling (CoMPP), S.K. Kračun, J.U. Fangel, M. G. Rydahl et al.,
• Analysis of Invertebrate and Protist N-Glycans, A. Hykollari, K. Paschinger, B. Eckmair, I. B. H. Wilson
• High-Throughput and High-Sensitivity Mass Spectrometry-Based N-Glycomics of Mammalian Cells, S. Holst, G.W. van Pelt, W.E. Mesker, R.A. Tollenaar, A.I. Belo, et al.,
• Analysis of N-Glycosylation of Total Membrane Proteins, T.Pavić & O. Gornik
• HILIC-UPLC Analysis of Brain Tissue N-Glycans, T. Klarić & I. Gudelj
• Automated Integration of a UPLC Glycomic Profile, A. Agakova, F. Vučković, L. Klarić, G. Lauc & F. Agakov
• Databases and Associated Tools for Glycomics and Glycoproteomics, F. Lisacek, J. Mariethoz, D. Alocci, P.M. Rudd, J.L. Abrahams et al.,
• High-Throughput N-Glycan Analysis with Rapid Magnetic Bead-Based Sample Preparation, M. Szigeti, A. Guttman