High-Throughput Glycomics and Glycoproteomics Methods and Protocols

The analysis of glycans is an often challenging endeavor, due to their heterogeneity, their non-template-based synthesis, and the abundance of functionally distinct isomers which are challenging for separation and characterisation using current analytical techniques.

Technological challenges in glycan analysis are generally seen as the major obstacle for revealing insights into functional relevance of glycans. Several robust methods that enable high-throughput analysis of glycans have been developed. Glycomics is now ready to join genomics and other omics in genetic, epidemiological, and clinical studies. This volume of Methods in Molecular Biology entitled High-Throughput Glycomics and Glycoproteomics brings detailed protocols for the analysis of glycosylation at the level of free glycans and glycopeptides, an essential reference for researchers planning to enter this rapidly evolving field.