Microdomain Formation Controls Spatiotemporal Dynamics of Cell-Surface Glycopropteins

Two processes govern the dynamics of proteins on the cell membrane. The diffusion inside the membrane results in lateral movements which are characterized by spatial dynamics. The temporal dynamics characterizes the incorporation and internalization kinetics of membrane –protein transportation to their destinations on the membrane after biosynthesis followed by a retraction back into the cell. The occurrence of microdomain formations at the cell surface exhibiting glycosylation protuberances markedly influences these spatiotemporal dynamics.

The microdomain formation on the spatiotemporal dynamics of glycosylated membrane proteins is investigated qualitatively and quantitatively by high resolution fluorescence microscopy and artificially mimicked by metabolic glycoprotein engineering.